目的 探讨大鼠活化型表皮生长因子受体（phosphorylated epidermal growth factor receptor，pEGFR）表达与脊髓压迫性损伤（compressed spinal cord injury，CSCI）后神经功能恢复的关系及可能的机制。 方法 制备大鼠脊髓压迫性损伤模型，运用BBB（Basso Beattie Bresnahan）评分观察动物解压后神经功能的恢复情况，免疫印迹（Western blotting，WB）检测pEGFR、total-caspase-3、active-caspase-3的表达变化，免疫荧光双标（double-labeling immunoflurescence）检测pEGFR-NG2+、active-caspase-3-NG2+细胞的变化。 结果 CSCI解压后，BBB评分随时间延长而逐渐增加；pEGFR的表达、pEGFR-NG2+细胞的数量随时间延长而逐渐增加并于解压后14 d达到高峰，而total-caspase-3、active-caspase-3的表达和active-caspase-3-NG2+细胞的数量在解压后即达到高峰并随时间延长逐渐降低。腹腔注射pEGFR抑制剂14 d后，pEGFR的表达、大鼠BBB评分较未注射pEGFR抑制剂组明显降低；total-caspase-3、active-caspase-3的表达和active-caspase-3-NG2+细胞显著增加。 结论 大鼠pEGFR的表达与CSCI解压后神经功能的恢复有关，其机制可能在于pEGFR参与了Caspase-3信号通路的调节。
Abstract
Objective To investigate the relationship between expression of phosphorylated epidermal growth factor receptor (pEGFR) and neural function recovery after decompression of compressed spinal cord injury(CSCI) in rats and clarify its possible mechanisms. Methods The CSCI model was established. The neural function recovery after decompression were monitored by the Basso, Beattie &Bresnahan(BBB) locomotor rating scale; the expression of pEGFR, total-caspase-3, active-caspase-3 were detected by Western blot; pEGFR+-NG2+(NG2+ cells are precursors to oligodendrocytes)，active-caspase-3+-NG2+ cells were detected by double-labeling immunefluorescence assay. Results The BBB scores gradually increased with time after decompression of CSCI. Meanwhile, the expression of pEGFR, number of pEGFR+-NG2+ cells were up-regulated and reached the peak at day 14, consistent with the changes of motor functions. total-caspase-3, active-caspase-3 and caspase-3-NG2+ cells immediately reached the peak after decompression and gradually decreased with time. After intraperitoneal injection of pEGFR inhibitor for 14 days, expression of pEGFR and BBB scores of rats were significantly lower than those of group without pEGFR inhibitor injection. The expression of total-caspase-3, active-caspase-3, caspase-3-NG2+ cells significantly increased. Conclusions The expression of pEGFR is related to process of neural function recovery after decompression of CSCI in rats, and its mechanism may be due to the involvement of pEGFR in the regulation of Caspase-3 signaling pathway.
关键词
脊髓压迫性损伤； 神经功能恢复； pEGFR； /
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Caspase-3
Key words
Compressed Spinal Cord injury; /
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Neural function recovery; /
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pEGFR; /
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Caspase-3
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基金
重庆市研究生科研创新项目（40040200100410），重庆市永川区科委课题（Ycstc，2016nc5023）
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